摘要
目的 探讨支气管哮喘急性发作期患者中医证候与免疫功能、口咽部菌群多样性的关系.方法 将105 例支气管哮喘急性发作期患者分为 3 组,风哮组 41 例,冷哮组 30 例,热哮组 34 例.采集并检测患者口咽部菌群、血液CD4+T细胞、CD8+T细胞、CD4+/CD8+.对 3 组患者CD4+T细胞、CD8+T细胞、CD4+/CD8+,口咽部菌群多样性指数(Shannon 指数、Richness指数、Pielou 指数、Simpson指数)进行比较.结果 3 组患者CD4+T细胞、CD4+/CD8+比较,差异均有统计学意义(P＜0.05).其中,冷哮组CD4+T 细胞、CD4+/CD8+均高于热哮组,差异具有统计学意义(P＜0.05).3 组患者在Richness指数、Pielou指数、Shannon指数、Simpson指数方面比较,差异均无统计学意义(P＞0.05).结论 支气管哮喘急性发作期患者中医证候在免疫功能、口咽部菌群多样性方面具有不同特点,可为中医临床辨证提供参考依据.
Abstract
Objective To explore the relationship between TCM patterns,immune function,and oropharyngeal flora diversity in patients experiencing acute exacerbations of bronchial asthma.Methods 105 patients with acute exacerbations of bronchial asthma were divided into 3 groups:Wind Asthma(n=41),Cold Asthma(n=30),and Heat Asthma(n=34).Oral and pharyngeal microbial samples were collected and assessed alongside blood CD4+T cells,CD8+T cells,and CD4+/CD8+ ratio.A comparative analysis was conducted among the three groups concerning CD4+T cells,CD8+T cells,CD4+/CD8+ ratio,and oral and pharyngeal microbial diversity indices(SHANNON,Richness,Pielou,SIMPSON).No statistically significant differences were found among the three groups regarding Richness,Pielou,Shannon,and Simpson indices(P＞0.05).Results Significant differences were observed in CD4+T cells and the CD4+/CD8+ ratio in the three groups(P＜0.05).Specifically,the CD4+T cells and CD4+/CD8+ ratio of the Cold Asthma group are higher than those of the Heat Asthma group,with statistically significant differences(P＜0.05).No statistically significant differences were found among the three groups regardingthe Richness,Pielou,Shannon,and SIMPSON indices(P＞0.05).Conclusion TCM patterns in patients experiencing acute exacerbations of bronchial asthma exhibit distinct characteristics in relation to immune function,and the diversity of oral and pharyngeal flora,providing valuable references for clinical differentiation in traditional Chinese medicine.
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