Study on extracting polyphenols from Vitis amurensis seeds through ultrasound-assisted method using ethanol
Objective:To establish the optimized ultrasound-assisted extraction process of polyphenols from Vitis amurensis seeds,and to provide technical reference for the use of grape seeds.Methods:With vitis amurensis seeds as raw material and ethanol as solvent,the extraction rates of polyphenols were detected under different conditions,such as different ethanol concentrations (20％,40％,60％,80％,and95％),extraction time (10,20,30,40,and60 min),extraction temperatures (25℃,35℃,45℃,55℃,and 65℃),material/liguid ratios (1 ∶ 4,1∶8,1∶10,and 1∶ 12),extraction times (1,2,3,4,and5)andultrasonic powers (50,60,80,100,and 120 w) by single factor experiment and orthogonal test.Results:The optimum parameters identified were as follows:ethanol concentration was 60 ％,extracting temperature was 50℃,extraction time was 30 min,ultrasonic power was 120 W,material/liquid ratio was 1 ∶ 8,and extracting times was 2.Under the optimal extraction conditions,the extraction rate of polyphenols from Vitis amurensis seeds was 11.71％,and the polyphenols concentration was 32.86％.Conclusion:Compared with traditional solvent extraction,ultrasound-assisted extraction of polyphenols from Vitis amurensis seeds has more advantages;the craft is simple,reasonable and efficient.
Vitis amurensis seedspolyphenolsultrasonic waveextractionorthogonal test
国家科技期刊平台
NSTL
万方数据
维普
