首页|不同剂量红光和乙烯利处理对采后芒果成熟及活性氧代谢的影响

不同剂量红光和乙烯利处理对采后芒果成熟及活性氧代谢的影响

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为了探明不同剂量红光照射及乙烯利处理对采后芒果成熟及活性氧代谢的影响,明确其在催熟中的使用剂量,以未进行任何催熟处理的芒果为对照(CK),分别采用300 lx(RL-300)和600 lx(RL-600)的红光以及400 mg/L(ETH-400)和1 200 mg/L(ETH-1200)乙烯利对'台农1号'芒果进行催熟处理,测定果实呼吸强度、乙烯释放量、活性氧含量及其酶促和非酶促清除能力相关指标,分析不同处理对芒果成熟和活性氧代谢的影响,筛选适宜的催熟方式。结果表明:RL-600和ETH-400处理对芒果的催熟效果较好,与CK相比,果实提前4d成熟,呼吸强度峰值分别提高了 18%和12%,乙烯释放量峰值分别提高了 7%和17%,过氧化氢含量峰值分别提高了 14。0%和9。6%,促进了抗坏血酸过氧化物酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶的活性与谷胱甘肽含量的上升,保持了活性氧(ROS)产生与清除系统的平衡;ETH-1200处理加速了芒果活性氧的累积,由于其抗坏血酸过氧化物酶及过氧化氢酶活性水平不足以清除过氧化氢导致芒果贮藏6d时便发生过熟腐烂现象;RL-300处理组芒果的ROS相关酶活性较低,故活性氧过氧化氢的产生与清除水平均较低,从而无法促使芒果成熟。综上,RL-600和ETH-400处理可有效促进芒果成熟,其ROS的产生与清除系统能够维持平衡。
Different Doses of Red Light and Ethephon Treatments on Postharvest Mangoes Ripening and Reactive Oxygen Metabolism
This study aimed to investigate the effects of different doses of red light irradiation and ethephon treat-ments on the ripening and reactive oxygen species metabolism of postharvest mangoes,and clarify the recommended dosage in ripening.Mangoes without any ripening treatments were used as control,and the 300 lx(RL-300)and 600 lx(RL-600)red light,400 mg/L(ETH-400)and 1 200 mg/L(ETH-1200)ethephon treatments were applied for ripening,respectively.Then the respiratory intensity,ethylene release,active oxygen contents and enzymatic and non-enzymatic scavenging abilities of fruits were determined,and the effects of different treatments on ripening and active oxygen metabolism of mangoes were analyzed,finally the suitable ripening method was screened.The results showed that RL-600 and ETH-400 treatments presented superior ripening effects on mangoes,maturing 4 days earlier compared to CK,increasing the peak values of respiration rates by 18%and 12%,the peak values of ethylene release by 7%and 17%,the peak values of hydrogen peroxide by 14%and 9.6%,which also improved the activities of ascorbate peroxidase,catalase and glutathione reductase and the contents of glutathione,and maintained the balance between reactive oxygen species(ROS)production and scavenging system.The ETH-1200 treatment accelerated the accumulation of ROS in mangoes.As the activity levels of ascorbate peroxidase and catalase were insufficient to remove H2O2,overripe rot occurred in mangoes after 6 days of storage.The ROS-related enzyme activities of mangoes in RL-300 treatment group were lower,resulting in the lower production and clearance levels of ROS,which could not promote mangoes ripening.In conclusion,RL-600 and ETH-400 treatments can effectively promote mangoes ripening,and the ROS generation and clearance system can maintain a balance.

mangoespostharvest ripeningreactive oxygen metabolismenzyme activitiesascorbate-glutathione cycle

孙宏图、刘帮迪、孙静、郭雪霞、郭淑珍、周新群、郝光飞、赵山山

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河北工程大学生命科学与食品工程学院,河北 邯郸 056038

农业农村部规划设计研究院,北京 100125

农业农村部产地初加工重点实验室,北京 100121

芒果 采后成熟 活性氧代谢 酶活性 抗坏血酸-谷胱甘肽循环

2025

保鲜与加工
国家农产品保鲜工程技术研究中心(天津)

保鲜与加工

北大核心
影响因子:0.956
ISSN:1009-6221
年,卷(期):2025.25(1)