Study on the Acid Tolerance Role of Transcription Factor RdrA in Lactococcus lactis F44
Boosting the acid resistance of microbial strains is of considerable economic importance in the industrial fermentation.Recent findings have shed light on an unexplored DeoR/GlpR family transcription factor,RdrA,which plays a pivotal role in enhancing the acid tolerance of Lactococcus lactis F44.To assess the impact of RdrA on the acid resistance of the L.lactis F44,a comparative analysis was conducted.This involved examining the growth and pH curves,as well as the acid and nisin resistance,and the nisin yield of both the rdrA knockout and overexpression strains in comparison to the wild-type F44 strains.Subsequently,transcriptomic sequencing was carried out on the rdrA knockout and wild-type F44 strains to identify differentially expressed genes and analyze acid resistance regula-tory mechanisms of RdrA.The transcriptomic data,corroborated by qPCR,indicated that RdrA's influence on the F44 strain's environmental tolerance was primarily due to its role in diminishing the fluidity and integrity of the cell envelope.Additionally,it was found that RdrA reduces intracellular methionine levels and impedes DNA repair and protein modification processes.This study unveils the unknown transcription factor RdrA and provides new insights and molecular targets for enhancing the acid tolerance of L.lactis,which could have significant implications for in-dustrial applications.
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