Research Progress in Allergens from Gluten and Their Detection and Analytical Methods
Gluten protein-induced allergic reactions have become a serious health problem and are recognized as one of the eight major allergens by the Food and Agriculture Organization of the United Nations and the World Health Organization.The Codex Alimentarius Commission stipulated that foods containing no more than 20 mg/kg of gluten can be labeled as gluten-free.Therefore,to ensure the life and health safety of consumers,it is essential to establish accurate and sensitive testing methods.This paper summarizes an overview of gluten allergens and currently used analytical methods,and discusses the advantages and disadvantages of enzyme-linked immunosor-bent assay,polymerase chain reaction,and liquid chromatography-tandem mass spectrometry,providing a refer-ence for further research on gluten detection techniques.