首页|凝乳酶对成熟牦牛乳硬质干酪氨基酸、脂肪酸及挥发性风味化合物的影响

凝乳酶对成熟牦牛乳硬质干酪氨基酸、脂肪酸及挥发性风味化合物的影响

扫码查看
为研究小牛皱胃酶、地衣芽孢杆菌D3。11凝乳酶和木瓜凝乳酶对成熟牦牛乳硬质干酪氨基酸、脂肪酸和挥发性风味化合物的影响,采用氨基酸分析仪、顶空固相微萃取和气相色谱-质谱联用等技术进行检测。结果表明,随着成熟时间的增加,3种干酪水分含量呈现下降趋势,TN,pH4。6-SN和12%TCA-SN含量呈现上升趋势,且TN与水分含量成负相关,其蛋白水解能力:BR>PR>CR;BR-YMHC中氨基酸含量显著高于其他2种干酪;3种干酪脂肪含量在成熟过程中始终呈现下降趋势,其脂肪降解能力:BR>PR>CR;与CR-YMHC相比,BR-YMHC和PR-YMHC中不饱和脂肪酸的相对含量较高,且功能脂肪酸(油酸)在BR-YMHC中相对含量最高;在3种干酪中分别鉴定出36,35和32种挥发性风味化合物,其中CR-YMHC中酮类物质相对含量最高,为26。39%;BR-YMHC中酯类物质相对含量最高,为43。02%;PR-YMHC中酸类物质相对含量最高,为31。86%。研究结果为酶凝干酪的生产加工及牦牛乳硬质干酪的风味调控提供理论依据。
Effects of rennets on amino acids, fatty acids and volatile flavor substances of ripe yak milk hard cheese
To investigate the effects of rennets on amino acids,fatty acids and volatile flavor compounds,amino acid analyzers, headspace solid-phase microextraction and gas chromatography-mass spectrometry were used to determine the contents and combinations of amino acids,fatty acids and volatile flavor compounds in Calf Rennet,Bacillus licheniformis D3.11 Rennet and Papain Rennet Yak Milk Hard Cheese. The results show that with the increase of ripening time,the moisture contents of the three types of cheese show a decreasing trend,while the contents of TN,pH4.6-SN,and 12%TCA-SN show an increasing trend,and TN is negatively correlated with moisture content. Their protein hydrolysis ability is BR>PR>CR;The amino acid content in BR-YMHC is significantly higher than the other two types of cheese;The fat contents of three types of cheese always show a decrease trend during the maturation process,and their fat degradation ability is BR>PR>CR. Compared with CR-YMHC,the relative content of unsaturated fatty acids in BR-YMHC and PR-YMHC is higher,and the relative content of functional fatty acids(oleic acid) is the highest in BR-YMHC;36,35,and 32 volatile flavor compounds were identified in three types of cheese,among which CR-YMHC has the highest relative content of ketones(26.39%);BR-YMHC has the highest relative content of esters(43.02%);and PR-YMHC has the highest relative content of acids(31.86%).The results provide a theoretical basis for the production and processing of enzymatic curd cheese and the flavor regulation of yak milk hard cheese.

rennetyak milk hard cheeseamino acidsfatty acidsflavor compounds

苗景源、乔海军、贾志龙、张倩、张卫兵、贾利蓉

展开 >

甘肃农业大学 理学院,兰州 730070

中国农业大学 营养与健康系,北京 100193

甘肃农业大学 食品科学与工程学院,兰州 730070

凝乳酶 牦牛乳硬质干酪 氨基酸 脂肪酸 风味化合物

国家自然科学基金国家自然科学基金国家自然科学基金

218640023206055231960486

2024

包装与食品机械
中国机械工程学会

包装与食品机械

CSTPCD北大核心
影响因子:1.019
ISSN:1005-1295
年,卷(期):2024.42(2)
  • 13