Isolation of Haloactinopolyspora alba and its degradation of polyethylene food preservation film
To solve the problem of difficult degradation of polyethylene(PE)packaging film,a strain from soil that can degrade PE plastic was isolated.After screening and purification,morphological observation,physiological and biochemical identification,and 16S rDNA sequence cloning were done to identify the strain D-1.Then the strain was inoculated on simple inorganic salt medium with PE film as the sole carbon source and cultured at 35℃,150 r/min.The results show that the strain D-1 is identified as Haloactinopolyspora alba.After 28 d incubation,the weight loss of PE film reaches 3.34％.Many holes and fissure were observed on the surface of PE film by SEM;and oxygen-containing functional groups such as carbon-carbon triple bond,ether bond and carboxyl group were introduced into the PE film through infrared scanning,and the laccase activity was 8.7 U/L.This study provides a scientific basis for the degradation of PE film,and enriches the resource bank of the degrading strains.
国家科技期刊平台
NSTL
万方数据
