包装与食品机械2024,Vol.42Issue(5) :24-33.DOI:10.3969/j.issn.1005-1295.2024.05.003

汉麻蛋白与EGCG复合物的制备及应用

The preparation and application of hemp protein and EGCG complex

李璐璐 杨杨 马春敏 王冰 刘晓飞 张光 边鑫 张娜
包装与食品机械2024,Vol.42Issue(5) :24-33.DOI:10.3969/j.issn.1005-1295.2024.05.003

汉麻蛋白与EGCG复合物的制备及应用

The preparation and application of hemp protein and EGCG complex

李璐璐 1杨杨 1马春敏 1王冰 1刘晓飞 1张光 1边鑫 1张娜1
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作者信息

  • 1. 哈尔滨商业大学食品工程学院,哈尔滨 150076
  • 折叠

摘要

为探究没食子酸酯(EGCG)对汉麻蛋白(HPI)功能特性与抗氧化活性的影响,通过单因素正交试验确定HPI-EGCG复合物的最佳制备条件,研究其功能特性和抗氧化能力,并评估其在植物蛋白饮料中的应用潜力.结果表明,在pH值为8,温度为60℃和盐浓度为0.2 mol/L的条件下,得到HPI-EGCG复合物最佳溶解度(53.87±0.42)%.与单独的HPI相比,HPI-EGCG复合物的乳化性、乳化稳定性、DPPH和ABTS自由基清除率分别提高110.38%,82.73%,59.44%,30.60%.在植物蛋白饮料的应用中,HPI-EGCG复合物表现出比大豆分离蛋白更好的离心沉淀率和稳定系数,在感官评价中获得较高评分.研究为HPI-EGCG的相关研究提供理论依据,拓展HPI和EGCG在食品工业中应用范围.

Abstract

In order to investigate the effects of epigallocatechin gallate(EGCG) on hemp protein isolate(HPI) functional properties and antioxidant activity,optimal preparation conditions for HPI-EGCG complexes were determined through single-factor orthogonal experiments.The complexes'functional properties,antioxidant capacity,and potential application in plant protein beverages were evaluated.Results show that the optimal solubility (53.87±0.42%) of HPI-EGCG complexes can be achieved at pH 8,60 ℃,and 0.2 mol/L salt concentration.Compared to HPI alone,HPI-EGCG complexes exhibit improvements in emulsifying capacity,emulsion stability,DPPH and ABTS free radical scavenging rates by 110.38%,82.73%,59.44%,and 30.60%,respectively.In plant protein beverage applications,HPI-EGCG complexes demonstrate superior centrifugal precipitation rates and stability coefficients compared to soy protein isolate,and receive higher sensory evaluation scores.The study provides theoretical basis for related research of HPI-EGCG and expands the applications of HPI and EGCG in the food industry.

关键词

汉麻蛋白/EGCG/功能特性/植物蛋白饮料

Key words

hemp protein/EGCG/function characteristics/plant protein beverage

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基金项目

国家自然科学基金项目(32372387)

出版年

2024
包装与食品机械
中国机械工程学会

包装与食品机械

CSTPCD北大核心
影响因子:1.019
ISSN:1005-1295
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