For the purpose of detecting Nosema bombycis by hybridization in situ, the probe( 1.2 kb) was obtained by PCR from rRNA gene of Nosema bombycis and labeled by random primer synthesis technique with 32P -dATP. The result showed that at least 296 spores were detected from single silkworm egg, and the spores were also detected from the larvae infected by Nosema bombycis for 4 days ,6 days and 10 days respectively.
Nosema bombycisBom byx mori eggsBombyx mori larvaHybridization in situDiagnosis
NETL
NSTL
维普
万方数据
