The shape and number of cotyledon are the important contents of mulberry morphological characters. Based on the investigation of the seedlings of 83 mulberry hybridized combinations, much phenotypic diversity of mulberry cotyledon was found. Mulberry cotyledon showed orbicular, elliptic, ovate and spindle shapes. Several kinds of abnormal cotyledons were also observed. Most mulberry seedlings produced 2 cotyledons generally, but polycotyledonous seedlings with 3 or 4 cotyledons were observed in 20 combinations in this experiment. The rate of polycotyledonous seedling was different among the combinations.