RAPD Analysis on Degenerate Strains of Beauveria bassiana Used in Biocontrol of Apriona germari
Beauveria bassiana is an important biocontrol strain against Apriona germari. The study about genetic mechanism of Beauveria bassiana degeneration would facilitate control of strain degeneration using biological engineering technology and enhance biocontrol efficiency. In this study, variations of the 1st, 6th, 12th, 14th, 16th, 18th and 20th generations of B. bassiana strain were analyzed at the gene level using RAPD technology. The results showed that each generation not only has the common bands, but also has additional bands or missing bands. Compared with the 1st and 6th generations, the genomic DNA of the 12th generation has changed obviously, and that of the 14th generation continued such change till the 16th, 18th and 20th generations of which genomic DNA was stabilized. Genetic distances among the tested 7 generations varied within the range of 0. 05 ~0. 60, showing obvious genetic difference which is consistent with analytical results on increase of mycelium growth, reduction of sporulation yield, and decline of pathogenicity to A. germari larvae with the increase of generation number. These results showed that RAPD technology can be used to conduct early genetic determination on degeneration of B. bassiana for biocontrol of A. germari larvae.
NETL
NSTL
万方数据
维普
