Mulberry root rot disease caused by the infection of fungal pathogen Lasiodiplodia theobromae is a newly discovered mulberry root disease in the sericultural regions of Guangxi.Investigation result of the relationships between disease occurrence and the age of the mulberry trees,the varieties and the occurrence of root-knot nematodes showed that,the older the mulberry trees was,the more serious the disease.The disease resistance varied between different mulberry varieties,among which Kangqing 283 and Qiangsang 1 have a stronger resistance.Root-knot nematodes were found in all diseased mulberry roots,but no symptoms of mulberry root rot disease were observed in some mulberry roots damaged by root-knot nematodes.Effects of illumination on the mycelia growth,the development of pycnidia and conidia,and the germination of conidia were studied.The results indicated that there was no significant difference in mycelia growth and pycnidia germination of L.theobromae under illuminated and dark conditions,but sustained illumination was beneficial to the development of pycnidia and conidia.The occurrence and damage of mulberry root rot disease caused by this new pathogen could be effectively reduced by improving field management,controlling the root-knot nematodes and planting mulberry varieties with high disease resistance.