An Analysis on Differentially Expressed Genes in Bombyx mori Fat Body with Injury Induced by Isoniazid Based on Digital Gene Expression Library
In this paper,using silkworm (Bombyx mori) as experimental animal,we studied the differential gene expression in injured fat body induced by isoniazid (INH) which is prescribed for as antituberculotics through high-throughput sequencing technique.At 8h after oral administration of INH to day 3 silkworm larvae of the 5th instar at dosage of 1 and 2 mg per larva,total RNA was extracted from fat body,and digital gene expression (DGE) libraries of fat body from INH treatment group and from blank control group (CK) were constructed using RNA-Seq sequencing technology.Comparative analysis on the differentially expressed genes between two DGE libraries indicated that INH treatment affected the expression of functional genes in fat body,especially the number of down-regulated genes was significantly higher.GO annotation and KEGG pathway analysis showed that these differentially expressed genes were enriched in nucleotide damage,extracellular signal transduction,cell apoptosis and oxidative stress,protein metabolism,drug metabolism and immunotoxicity etc.We randomly selected 4 differentially expressed genes involved in hepatic injury,drug metabolism and mitochondrial energy metabolism to perform qRT-PCR detection,and the results were consistent with that in DGE library.Observation of fat body after HE and DAPI staining also verified that INH could induce cell injury.Activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST),which are related to liver damage,in fat body of silkworm at different time points after treatment with INH of different concentrations were determined,and the results showed that they were all significantly increased and had different variation tendency.It is speculated that the binding of INH to relevant macromolecules affected various metabolic pathways with involvement of these macromolecules,and resulted in tissue injury.
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万方数据
维普
