Effect of Mulberry Leaf 1-Deoxynojirimycin on Glucose and Lipid Metabolism in Hyperlipidemia Model Mice
In order to develop and utilize the biological activity of 1-deoxynojirimycin (DNJ),a polyhydroxyl piperidine alkaloid from mulberry leaf,the effect and its underlying mechanism of DNJ on glucose and lipid metabolism were observed in hyperlipidemia model mice induced with high-fat diet.After the self-made freeze-dried DNJ powder of mulberry leaf was administered to hyperlipidemia model mice for 90 d at 50 mg/(kg·d) dosage,postprandial blood glucose,total cholesterol (TG),insulin level and insulin resistance index of the treated mice were extremely significantly decreased (P＜0.01).Meanwhile,the activities of glucokinase (GK) and pyruvate kinase (PK) and the contents of apolipoproteins (APO-A1 and APO-B100) were extremely significantly increased (P＜0.01).qRT-PCR results showed that the transcriptional levels of glucose and lipid transportation key proteins,namely,APO-A1,APO-B100,lipoprotein lipase (LPL),endoplasmic reticulum acyl coenzyme A synthetase (AcylCoA-S),glycerol kinase (Gly-K) and 3-phosphate dehydrogenase (G3PD),were also extremely significantly up-regulated in mouse liver tissues.Western blot analysis displayed that two gluconeogenesis enzymes,pyruvate carboxylase (PCB) and phosphoenolpyruvate carboxykinase (PEPCK),were down-regulated.Hepatic histopathological analysis revealed that hepatic cords were degenerated and lipid droplets were markedly increased in liver cells of hyperlipidemia model mice.After treatment with mulberry leaf DNJ,lipid droplets were significantly reduced in mouse liver cells.These results suggest that DNJ could decrease blood glucose and lipid levels in hyperlipidemia model mice via regulating the expression of enzymes involved in glucose and lipid metabolism,thereby reducing liver injury induced by high-fat diet.
Mulberry leaf1-DeoxynojirimycinHyperlipidemia model miceFatty liverGlucose metabolismLipid metabolism
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NSTL
万方数据
维普
