The insecticide acetamiprid is widely used in controlling agricultural pests.The acute toxicity and chronic toxicity of acetamiprid to silkworm (Bombyx moii) were tested by using food intake method.Results of acute toxicity bioassay showed that 96 h median lethal concentration (LC50) of acetamiprid to the 3rd,the 4th and the 5th instar were 0.361,0.692 and 1.339 mg/L,respectively,indicating that acetamiprid is a high-risk or ultra-high-risk grade poison to silkworm.After treating the 3rd instar newly exuviated silkworm larvae continuously with low concentration of acetamiprid,the duration of each instar was prolonged,and the body weight of moulting or matured larvae,cocoon weight,cocoon shell weight and cocoon shell rate were all adversely affected.In addition,when the 3rd instar newly exuviated larvae were treated with acetamiprid of ≥ 0.011 mg/L,the superoxide dismutase (SOD) activity in larval midgut of day 6 of the 5th instar larvae was significantly activated (P ＜ 0.05),while the catalase (CAT) activity was markedly inhibited (P＜0.05).The activity of glutathione peroxidase (GSH-Px) was improved by low concentration (0.005-0.022 mg/L) of acetamiprid but was inhibited by high concentration (0.045 and O.090 mg/L) of acetamiprid.The results indicate that acetamiprid has extremely high risk to sericultural production and should be used in sericultural areas with caution Under high concentration,although SOD activity of silkworm larvae was activiated,but CAT and GSH-Px activities of silkworm larvae were both inhibited.This is the reason that acetamiprid can do harm to silkworm growth and development and to cocoon production.
AcetamipridBombyx moriLC50Growth and developmentAntioxidant enzyme activity
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