Genome Cloning and Transmission Manner Analysis of a New Citrus Leaf Blotch Virus Isolate from Mulberry
Citrus leaf blotch virus (CLBV) has the potential to infect a wide range of species. In 2018, CLBV was detec-ted in wrinkled and narrowed mulberry leaves through next-generation sequencing. The complete genome sequence of mulberry isolate of CLBV ( CLBV-MA) was determined by the combination of RT-PCR, 5′-and 3′-RACE. It is 8617 nucle-otides long (excluding the polyA tail), which contains three open reading frames encoding replicase proteins (RP), movement protein ( MP) and coat protein ( CP) , respectively. The amino acid sequence identities of RP and CP between CLBV-MA and other isolates in the genus Citrivirus are 79. 0％to 89. 1％and 87. 6％to 94. 7％, respectively, suggesting that CLBV-MA is a new isolate. Phylogenetic analysis based on whole genomic nucleotide squences revealed that CLBV-MA, CLBV-Actinidia, CLBV-HZ, CLBV-XL, CLBV-Actinidia-V20 and CLBV-ML cluster into a branch, indicating that they are genetically related to each other. Additionally, CLBV-MA dates back further than others in this branch. Depending on the propagation mode, pruning management, age, and distribution of CLBV-MA in mulberry field, CLBV-MA is transmitted mainly through grafting and at a low rate by contaminated knives. The determination of the complete genome sequence of CLBV-MA and its transmission manner lays the foundation to develop molecular detection methods and formulate effective prevention and control strategies.
MulberryCitrus leaf blotch virusComplete genome sequenceMode of transmission
万方数据
维普
