Isolation, Identification and High-Throughput Sequencing Analysis of the Pathogenic Fungus of Mulberry Brown Spot Disease
Mulberry brown spot disease (MBSD) is a common fungal disease. In this study, samples of MBSD were collected from the main sericultural area in Ankang City, Shaanxi Province. Combined with Koch 's postula-tes, morphological and molecular biology methods were used to isolate and identify the pathogenic fungus, and high-throughput sequencing technology was used to determine the main groups of pathogenic fungi. Furthermore, the colonization state of pathogenic fungi in mulberry leaves was observed by chloral hydrate clearing reaction. The results showed that the pathogen of MBSD infecting mulberry trees in Ankang, Shaanxi Province was Neophloeospora mac-ulans. The pathogenic fungi grow slowly on PDA medium and produce white conidia, which are transparent cylindrical with tapering at both ends and septum. The hyphae mainly parasitizes around the stomates and leaf veins of mulberry leaf. The rDNA sequence of the pathogen has a total length of 5512 bp and a GC ratio of 51. 16％. Based on above results, the mulberry brown spot pathogens infecting the main sericultural area of Ankang, Shaanxi Province were deter-mined. The research results provide a theoretical basis for the prevention and control of MBSD.
Mulberry fungal diseaseMulberry brown spot diseaseNeophloeospora maculansHigh-throughput sequencing
万方数据
维普
