Expression Pattern of Detoxification Enzyme Genes in Silkworm Variety Fu 7 Under Deltamethrin Stress
Overexpression and increased activity of detoxification enzyme in insects are the main reasons for insect re-sistance to insecticides. Radiation induced silkworm variety Fu 7 has strong tolerance to pyrethroid pesticides. Studying the expression pattern of detoxification enzyme genes of Fu 7 under deltamethrin stress is of great significance for analyzing the pesticide tolerance mechanism of Fu 7 and breeding silkworm varieties resistant to pesticide pollution. According to the observation of courtship and mating be-havior of male moths of Fu 7 treated with different concen-trations of deltamethrin, the 2. 5 mg/L deltamethrin treat-ment group was finally selected to detect the expression of detoxification enzyme genes. Fluorescence quantitative PCR was used to detect the transcriptional level of the antennal detoxification enzyme genes ( 8 carboxylesterase genes, 2 serine protease inhibitor genes, 1 chymotrypsin gene, 3 lipase genes, 1 cytochrome P450 gene and 1 glutathi-one-S-transferase gene) of Fu 7 male moth after 3 and 6 h of deltamethrin treatment:the transcriptional level of 7 carbox-ylesterase genes was first up-regulated and then down-regulated, and the transcriptional level of 1 carboxylesterase gene was first down-regulated and then up-regulated;the transcriptional level of one cytochrome P450 gene, one serine protease inhibitor gene and one chymotrypsin gene were up-regulated and then down-regulated;the transcriptional level of one glutathione S-transferase gene, one serine protease inhibitor gene and two lipase genes were continuously up-reg-ulated;the transcriptional level of one lipase gene was up-regulated after 6 h. The results showed that the transcriptional level of detoxification enzyme genes in Fu 7 male moth were up-regulated after deltamethrin treatment, which enhanced the tolerance of male Fu 7 moth to micro pyrethroid pesticides to some extent.
万方数据
维普
