Regulating Function of N-Glycan Biosynthesis Pathway Genes ER 1,2-Alpha-Mannosidase and Alpha-Mannosidase Ⅱ on Embryonic Diapause in Bombyx mori
Diapause of the silkworm, Bombyx mori is the result of the synergy of genes and environment, and its regulatory network is very complicated and has not been fully elucidated yet.To better understand whether the N-glycan biosynthesis pathway of the silkworm is involved in the regulation of embryonic diapause, the transcription levels of BmMAN1B and BmMAN2 were analyzed in dif-ferent tissues of different voltinism silkworm strains during the pupal stage and early embryonic development stage, and in BmN cells after treating of diapause hormone (DH) or 20-hydroxyecdysone (20E).The qRT-PCR results showed that the transcription levels of the two genes varied greatly among different voltinism strains, with higher levels observed in the late pupal stage compared to that of the early pupal stage.When BmN cells were treated with 100 nmol/L DH and 10 ng/mL 20E respectively, the transcription levels of BmMAN1B and BmMAN2 genes were significantly upregulated, suggesting that both BmMAN1B and BmMAN2 were closely related to the diapause process of the silkworm.The changes of transcription levels of diapause-related genes in-cluding BmDH, BmDHR-1, BmTRE2 and BmSDH-2a were detected post RNA interference ( RNAi) of BmMAN1B and BmMAN2 in BmN cells and in day-2 female pupae developed from bivoltine silkworm variety Qiufeng eggs incubated in 16.5 ℃( QFLT) , respectively.RNAi targeting both of BmMAN1B and BmMAN2 in individuals resulted in a shift of 38.2％progeny eggs from non-diapause to diapause, indicating that BmMAN1B and BmMAN2 play important regulatory roles in the diapause of Bombyx mori embryos.
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