Study on the protective effect of armillariella oral solution on colon cancer,melanoma metastasis and sarcoma
Objective To explore the effects of armillariella polysaccharide in armillariella oral solution on the growth,proliferation and invasion of malignant tumors such as colon cancer,melanoma and sarcoma.Methods In vitro,different concentrations(10,100 and 250 μg·mL-1)of armillariella polysaccharide(extracted from armillariella oral solution)were added to human colon cancer cell line HCT116 cells and mouse metastatic melanoma cell line B16-F10 cells.MTT assay was used to detect the cytotoxic effect of armillariella polysaccharide on colon cancer and melanoma.24 BALB/c female mice were selected at the in vivo level and randomly divided into the sarcoma-bearing control group(sarcoma ascites tumor cells with 2× 106cells/500μL were subcutaneously implanted into the left axilla of mice),the low-dose armillariella polysaccharide intervention group(intraperitoneal injection of 25 mg·kg-1 armillariella polysaccharide)and the high-dose armillariella polysaccharide intervention group(intraperitoneal injection of 50 mg·kg-1 armillariella polysaccharide).The effects of different concentrations of armillariella polysaccharide on peripheral blood biochemical indicators and pathological morphology of liver-kidney tissues in tumor-bearing mice were analyzed.Results 10,100 and 250 μg·mL-1 of sulfated armillariella polysaccharide could not significantly inhibit the growth of the above tumor cells(P＞0.05).In the low-dose armillariella polysaccharide intervention group and the high-dose armillariella polysaccharide intervention group,the inhibitory effects on tumor growth were stronger than those in the sarcoma-bearing control group,and the induction of increased spleen weight,peripheral blood platelet count and AST levels in tumor-bearing mice was higher than that in the sarcoma-bearing control group(P＜0.05).Conclusion At the in vivo level,armillariella polysaccharide can delay the growth and proliferation of sarcoma in tumor-bearing mice,and it has no significant hepatorenal toxicity on tumor-bearing mice,and has little effect on the growth of colon cancer and melanoma.
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