Study on the mechanism of circ_0000515 regulating proliferation,migration and invasion of ovarian cancer SKOV3 cells by targeting miR-924
Objective To explore the molecular mechanism of circ_0000515 regulating the proliferation,migration and invasion of ovarian cancer SKOV3 cells.Methods qRT-PCR method was used to detect the expression levels of circ_0000515 and miR-924.Human ovarian cancer cell SKOV3 was cultured in vitro.CCK-8 method,plate clone formation test,scratch test and Transwell test were used to analyze the proliferation,clone formation,migration and invasion of the cell.The relationship between miR-924 and circ_0000515 was confirmed by dual luciferase reporter experiments.Western blot was used to detect protein expression levels.Results Compared to paracancerous tissue,circ_0000515 expression was upregulated in ovarian cancer tissue,while miR-924 expression was downregulated(P＜0.05).Compared with the si-NC group,the cell viability and the number of cell clones in the si-circ 0000515 group were decreased(P＜0.05).Compared with the si-NC group,in the si-circ_0000515 group,the scratch healing rate,number of invasive cells,and N-cadherin protein level decreased,while the E-cadherin protein level increased,and there was statistically significant difference(P＜0.05).Compared with the miR-NC group,WT-circ 0000515 expression in the miR-924 group decreased(P＜0.05).Compared with the pcDNA group,mir-924 expression in the pcDNA-circ 0000515 group was increased,while compared with the si-NC group,miR-924 expression in the si-circ 0000515 group was increased,and there was statistically significant difference(P＜0.05).Compared with the miR-NC group,the cell viability,scratch healing rate,and N-cadherin protein level in the miR-924 group decreased,while the number of cell clones and invasive cells decreased,and the E-cadherin protein level increased(P＜0.05).Compared with the si-circ 0000515+anti miR-NC group,the cell viability,scratch healing rate,and N-cadherin protein level were increased in the si-circ 0000515+anti miR-924 group;The number of cell clones and invasive cells increased,while the E-cadherin protein level decreased(P＜0.05).Conclusion Circ_0000515 silence inhibits the malignant behavior of ovarian cancer cells by targeting miR-924.
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