Regulation mechanism of cytokines involvement in neuropathic pain treated by Xinrun Xuantong method
Objective To analyze the regulatory mechanism of cytokines involvement in neuropathic pain treated by Xinrun Xuantong method.Methods A total of 120 SPF Wistar rats were selected and divided into six groups,namely the sham operation group,the model group,Xuanfuhua decoction high-dose group,medium-dose group,low-dose group,and pregabalin group,with 20 rats in each group.The sham operation group was fed normally,and the remaining five groups were established neuropathic pain rat models.After successful modeling,Xuanfuhua decoction high-dose group,medium-dose group and low-dose group were given 10 g·kg1,5 g·kg-1,and 2.5 g·kg-1 Xuanfuhua decoction respectively,pregabalin group was given 15 mg·kg-1 pregabalin solution,the sham operation group and the model group were given the same amount of 0.9％sodium chloride solution,once a day,and after 15 days,the changes of rats were observed.Results Compared with the sham operation group,spontaneous pain score,the levels of interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),5-hydroxytryptamine(5-HT)and prostaglandin E2(PGE2),and expression levels of Toll like Receptor 4(TLR4)and nuclear factor-κB(NF-κB)mRNA increased in the model group,pregabalin group,Xuanfuhua decoction low-dose group,medium-dose group and high-dose group,and the levels of mechanical withdrawal threshold(MWT),thermal withdrawal latency(TWL),IL-10 and β-endorphin(β-EP)were decreased(P＜0.05);Compared with the model group,spontaneous pain scores,levels of IL-1β,TNF-α,5-HT and PGE2,and expression levels of TLR4 and NF-κB mRNA in pregabalin group,Xuanfuhua decoction low-dose group,medium-dose group and high-dose group were decreased,and the levels of MWT,TWL,IL-10 and β-EP were increased(P＜0.05);Compared with pregabalin group,Xuanfuhua decoction low-dose group and medium-dose group,the spontaneous pain score,levels of IL-1β,TNF-α,5-HT and PGE2,and expression levels of TLR4 and NF-κB mRNA in Xuanfuhua decoction high-dose group were decreased,and the levels of MWT,TWL,IL-10 andβ-EP were increased(P＜0.05).Conclusion The levels of inflammatory factors in neuropathic pain rats are regulated after treatment with Xinrun Xuantong method,pain is reduced,and the sensitivity to mechanical and thermal stimulation is improved in a dose-dependent manner.The mechanism may be related to the inhibition of TLR4/NF-κB signaling pathway.
万方数据
维普
