Study on the effect and mechanism of rubidium minerals in inhibiting the proliferation and metastasis of lung cancer in mice
Objective To investigate the effect and mechanism of rubidium minerals in inhibiting the proliferation and metastasis of lung cancer in mice.Methods Mice were injected subcutaneously with Lewis lung cancer(LLC)cells to construct the animal model of lung cancer.And after 14 days of drug intervention,the size of tumors,blood routine,inflammatory factors in serum and tumor,and reactive oxygen species(ROS)expression in mice were measured;Histopathological changes in tumor,heart,liver,spleen,lung,kidney were observed.Mouse tail vein injection of B16-F10 cells was used to construct melanoma lung cancer metastasis model,and blood routine changes were detected.The lung wet weight and its pathological changes were observed.Results In the experiment on the effect of rubidium minerals on mice transplanted lung cancer,after 14 days of drug(rubidium minerals or Gefitinib)intervention,the contents of white blood cell(WBC)and lymphocyte(Lym)in mice were lower than those in the model control group(P＜0.01);And the volume and weight of the solid tumor were relatively small,the number of tumor cells in tumor tissues decreased,the nucleus shrank,and different degrees of necrosis occured;There was no significant damage to the heart,liver,lungs,or kidneys;Spleen injury was mild,spleen weight decreased,serum levels of interleukin,(IL)-6 and ROS increased,and tumor necrosis factor-α(TNF-α)content decreased;In tumor tissue,the levels of IL-1β and IL-6 decreased,and TNF-α content increased(P＜0.05).In the experiment on the effect of rubidium minerals on lung cancer metastasis in mice,after 18 days of drugs(rubidium minerals or Gefitinib)intervention,the contents of neutrophils(Neu)and WBC in mice were significantly reduced,lung wet weight was significantly decreased(P＜0.001),there were few lung metastases,and inflammatory response and lung metastasis of malignant melanoma were significantly inhibited.Conclusion Rubidium minerals can significantly inhibit the proliferation and metastasis of lung cancer in mice,and its mechanism of action may play the role in the treatment of lung cancer by regulating the immunity of the body,reducing the levels of inflammatory factors,and inhibiting ROS production.
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