Objective To establish a method for determining the contents of psoralen and isopsoralen in Wenshen Nuanpi Granules in order to provide experimental basis for quality control of Wenshen Nuanpi Granules.Methods Octadecylsilane bonded silica gel was used as filling agent.The mobile phase was methanol∶water(40∶60 v/v).The detected wavelength was 246 nm.Results Linear-relationship between peak area and mass concentration was good for psoralen(r2=0.9992)and isopsoralen(r2=0.9993);The precision and stability were in accordance with the requirements;The average recovery rate of psoralen was 98.25％with an RSD of 0.89％,and the average recovery rate of isopsoralen was 95.90％with an RSD of 1.18％.Conclusion In this experiment,the high performance liquid chromatography method for determining the content of psoralen and isopsoralen is investigated and established,which can be used as the basis for the quality control of Wenshen Nuanpi Granules.
维普
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