Effects of Gegenqinlian decoction on glycolipid metabolism of diabetes mellitus type 2 rats with pattern of dampness-heat in stomach and intestine through IRS-1/PI3K/AKT pathway
Objective To explore the effects of Gegenqinlian decoction on glycolipid metabolism of diabetes mellitus type 2 rats with pattern of dampness-heat in stomach and intestine through insulin receptor substrate-1(IRS-l)/phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)pathway.Methods 40 SD rats were randomly divided into the normal group(2mL of normal saline by gavage),the modeling group(2mL of normal saline by gavage),the metformin group(4.17mg/100g metformin by gavage),and the Gegenqinlian decoction group(lg/100g Gegenqinlian decoction by gavage),with 10 rats in each group.The diabetes mellitus type 2 rat model was established with high-fat and high-sugar diet and intrabitoneal injection of streptozotocin(STZ),and then diabetes mellitus type 2 rat model with pattern of dampness-heat in stomach and intestine was established by feeding oil,42° white wine and honey water.The body weight of each group was measured at different time points,and the level of fasting blood glucose(FBG)was measured by glucose meter.The levels of fasting insulin(FINS),triglyceride(TG),total cholesterol(TC),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by ELISA,and insulin resistance index(HOMA-IR)was calculated.The histopathological changes of liver were detected by HE staining.The changes of catalase(CAT),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and malondialdehyde(MDA)levels in liver tissue were detected.The changes of IRS-1,PI3K,p-PI3K,AKT and p-AKT protein contents in liver tissue were detected by Western blot.Results Compared with the normal group,body weight,and levels of FBG,FINS,HOMA-IR,GSH-Px,CAT,SOD,IRS-1,p-PI3K/PI3K and p-AKT/AKT in the modeling group were significantly decreased(P＜0.05),the levels of TG,TC,IL-6,TNF-α and MDA in the modeling group were significantly increased(P＜0.05),indicating focal hepatic parenchymal loss.Compared with the modeling group,body weight,and the levels of FBG,FINS,HOMA-IR,GSH-Px,CAT,SOD,IRS-1,p-P13K/PI3K and p-AKT/AKT in the metformin group and Gegenqinlian decoction group were significantly increased(P＜0.05),while the levels of TG,TC,IL-6,TNF-α and MDA in the metformin group and Gegenqinlian decoction group were significantly decreased(P＜0.05),indicating normal hepatic parenchyma.Conclusion Gegenqinlian decoction can significantly improve the glucolipid disorder of diabetes mellitus type 2 rats with pattern of dampness-heat in stomach and intestine,which may play the role through IRS-1/PI3K/AKT pathway.
Gegenqinlian decoctionpattern of dampness-heat in stomach and intestinediabetes mellitus type 2glycolipid metabolisminsulin receptor substrate-1/phosphatidylinositol 3 kinase/protein kinase pathway
万方数据
维普
