Objective To explore the mechanism of pravastatin on improving placental oxidative stress in rats with preeclampsia(PE).Methods 27 PE rats were randomly divided into PE group,high-dose pravastatin group and low-dose pravastatin group,with 9 rats in each group.10 healthy pregnant rats were used as the control group.High-dose pravastatin group and low-dose pravastatin group were given pravastatin 50 mg·kg-1 and 25 mg·kg-1 per day,respectively.The control group and PE group were injected with the equal amount of normal saline.Blood pressure and 24-hour urine protein were measured.Placental and fetal body masses of rats were detected.The activities of serum superoxide dismutase(SOD)and catalase(CAT)were detected.The expression levels of hypoxia inducible factor 1α/heme oxygenase 1(HIF-1α/HO1)pathway related proteins in placental tissue were compared.Results Compared with PE group,in high-dose pravastatin group and low-dose pravastatin group,diastolic blood pressure(DBP),systolic blood pressure(SBP),and 24-hour urinary protein levels decreased,and the body mass,SOD and CAT levels,and the expression levels of nuclear factor E2-related factor 2(Nrf2),HIF-1α,HO-1 and vascular endothelial growth factor(VEGF)increased(P＜0.05).Compared with low-dose pravastatin group,in high-dose pravastatin group,DBP,SBP,and 24-hour urinary protein levels decreased,and the body mass,SOD and CAT levels,and the expression levels of Nrf2,HIF-1α,HO-1 and VEGF increased(P＜0.05).Conclusion Pravastatin can alleviate the symptoms of PE rats,reduce oxidative stress of PE rats,and its mechanism may be related to the regualtion of the HIF-1α/HO-1 signaling pathway.
维普
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