Study on the mechanism of pravastatin on improving placental oxidative stress in preeclampsia rats
Objective To explore the mechanism of pravastatin on improving placental oxidative stress in rats with preeclampsia(PE).Methods 27 PE rats were randomly divided into PE group,high-dose pravastatin group and low-dose pravastatin group,with 9 rats in each group.10 healthy pregnant rats were used as the control group.High-dose pravastatin group and low-dose pravastatin group were given pravastatin 50 mg·kg-1 and 25 mg·kg-1 per day,respectively.The control group and PE group were injected with the equal amount of normal saline.Blood pressure and 24-hour urine protein were measured.Placental and fetal body masses of rats were detected.The activities of serum superoxide dismutase(SOD)and catalase(CAT)were detected.The expression levels of hypoxia inducible factor 1α/heme oxygenase 1(HIF-1α/HO1)pathway related proteins in placental tissue were compared.Results Compared with PE group,in high-dose pravastatin group and low-dose pravastatin group,diastolic blood pressure(DBP),systolic blood pressure(SBP),and 24-hour urinary protein levels decreased,and the body mass,SOD and CAT levels,and the expression levels of nuclear factor E2-related factor 2(Nrf2),HIF-1α,HO-1 and vascular endothelial growth factor(VEGF)increased(P＜0.05).Compared with low-dose pravastatin group,in high-dose pravastatin group,DBP,SBP,and 24-hour urinary protein levels decreased,and the body mass,SOD and CAT levels,and the expression levels of Nrf2,HIF-1α,HO-1 and VEGF increased(P＜0.05).Conclusion Pravastatin can alleviate the symptoms of PE rats,reduce oxidative stress of PE rats,and its mechanism may be related to the regualtion of the HIF-1α/HO-1 signaling pathway.
万方数据
维普
