Objective To screen the inhibitors of angiotensin converting enzyme 2(ACE2)and dipeptidyl peptidase 4(DPP-4)from baical skullcap root.Methods ACE2 and DPP4 were used as the target proteins,the compounds in baical skullcap root that were compatible with ACE2 and DPP4 were screened and analyzed by receptor-ligand affinity ultrafiltration and liquid chromatography-mass spectrometry,in order to screen compounds that could simultaneously inhibit ACE2 and DPP-4.Results 5,7,2',6'-4 hydroxyflavone,5,7,2',5'-4 hydroxy-8,6'-dimethoxyflavone,chrysin-7-O-glucuronide,baicalein-6-O-glucuronide,oroxylin A-7-O-glucuronide,wogonoside,chrysin,oroxylin A were screened from baical skullcap root,and their affinity and inhibition towards ACE2 and DPP-4 coexisted,and they had potential anti novel coronavirus effect.Conclusion The target protein inhibitors related to novel coronavirus pneumonia screened from baical skullcap root have potential development value.
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