Effect of modified Taohong Siwu decoction on deep vein thrombosis formation after fracture in rats by inhibiting the formation of neutrophil extracellular traps
Objective To explore the effect of modified Taohong Siwu decoction on deep venous thrombosis after fracture in rats by inhibiting the formation of neutrophil extracellular traps(NETs).Methods A total of 24 SD rats were randomly divided into the blank control group,the model control group,the positive drug group and the Chinese medicine group,with 6 rats in each group.Rat model of femoral fracture with mechanical injury was prepared.The blank control group and the model control group received normal saline by gavage,the positive drug group received rivaroxaban tablets,and the Chinese medicine group administered 1 mL.100 kg-1 of rat body weight by gavage according to the formula.Blood sampls were taken from the eyeball at 7 days after modeling,and the levels of anticoagulant indicators in each group were detected.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),citrullinated histone H3(Cit-H3),myeloperoxidase(MPO)and D-dimer(DD)were detected by Enzyme-Linked Immunosorbent Assay(ELISA).The serum levels of superoxide dismutase(SOD)and malondialdehyde(MDA)were measured.The HE staining morphology of the left femoral vein tissue specimen was observed.Results Compared with the blank control group,serum levels of activated partial thromboplastin time(APTT),prothrombin time(PT)and thrombin time(TT)in the model control group decreased,and level of D-dimer(DD)increased(P<0.01).Compared with the model control group,serum levels of APTT,PT and TT in the positive drug group and the Chinese medicine group increased,and the level of DD decreased(P<0.05 or P<0.01).Compared with the blank control group,the serum SOD activity level in the model control group decreased,and MDA content increased(P<0.01).Compared with the model control group,the serum SOD activity level in the positive drug group and the Chinese medicine group increased,and MDA content decreased(P<0.05 or P<0.01).Compared with the blank control group,the serum levels of IL-6 and TNF-α in the model control group increased(P<0.01).Compared with the model control group,the levels of IL-6 and TNF-α in the positive drug group and the Chinese medicine group decreased,and the Chinese medicine group was lower than the positive drug group(P<0.05 or P<0.01).Compared with the blank control group,the serum level of MPO in the model control group increased,and the level of Cit-H3 decreased(P<0.01).Compared with the model control group,in the positive drug group and the Chinese medicine group,the serum levels of MPO and Cit-H3 decreased,and the Chinese medicine group was lower than the positive drug group(P<0.01).In the blank control group,the morphology and structure of the veins were normal,and there was no inflammatory cell infiltration.In the model control group,thrombosis could be seen,with damage to the vascular intima,the phenomenon of endothelial cell detachment in blood vessels,and inflammatory cell infiltration in the vascular wall.In the positive drug group,thrombus could still be seen,but it was smaller than that in the model control group.The thrombus in the Chinese medicine group was smaller than that in the model control group.Conclusion Modified Taohong Siwu decoction has the preventive and therapeutic effect on the formation of deep venous thrombosis in rats,and its mechanism may be to inhibit inflammation by inhibiting the formation of NETs,thereby intervening in the formation of thrombosis.
deep venous thrombosisneutrophil extracellular trapsinflammatory factorcoagulation function
万方数据
维普
