Effect of acupuncture on the expression of brain-derived neurotrophic factor and tyrosine kinase receptors B proteins in the hippocampus of rat model of post-stroke cognitive impairment
Objective To observe the effect of acupuncture on the brain-derived neurotrophic factor(BDNF)/tyrosine kinase receptors B(TrKb)signaling pathway in the hippocampus of rat model of post-stroke cognitive impairment(PSCI),and to explore the improvement effect of acupuncture therapy on PSCI and its possible mechanism of action.Methods 8 SD male rats were randomly selected as the sham-operated group(Sham group)from 40 SD male rats,while the remaining rats were used to establish the model of right middle cerebral artery occlusion(MCAO)using suture-occluded method.After modeling,the neurological function status of rats was assessed using the Zea-Longa score,and cognitive impairment rats were screened using Morris water maze.And then successfully modeled ras were randomly divided into the model group(MCAO group)and the acupuncture group(MAS group).The MAS group received acupuncture treatment at Baihui,Shenting,Fengfu,and Shenmen points,once a day,15 minutes each time,6 times per week,for a total of 28 days.The Sham group and MCAO group were only touched by hands,and then were placed back in their cages.Results The results of Zea-Longa score and Morris water maze test showed that the improvement in neurological deficits and learning and memory abilities in the MAS group was more significant than that in the MCAO group(P<0.05);HE staining results showed that compared with the MCAO group,the MAS group had reduced degeneration of neurons,compact tissue structure,and increased number of neurons.The results of RT-PCR showed that compared with the MCAO group,the expression levels of BDNF and TrKb in the MAS group were increased(P<0.05).Conclusion Acupuncture can improve the learning and memory abilities of PSCI model rats,and its mechanism may be related to the promotion of the BDNF/TrKb signaling pathway.
万方数据
维普
