Exploration of the mechanism of liver injury caused by ethanol extract of Inonotus Obliquus based on liver metabolomics
Objective To explore the potential mechanism of action of the ethanol extract of Inonotus Obliquus on rat liver injury using liver metabolomics technology.Methods 40 SD rats were randomly divided into the control group,and the high-dose group(2.0 g·kg-1),the medium dose group(1.0 g·kg-1)and the low-dose group(0.5 g·kg-1)of the ethanol extract of Inonotus Obliquus.The high-dose group,the medium dose group and the low-dose group of the ethanol extract of Inonotus Obliquus were administered by gavage once a day for 12 consecutive weeks.The control group was given pure water by gavage for 12 consecutive weeks.Metabolomics analysis was performed on the liver of rats in each group by ultra-high performance liquid chromatography-time of flight tandem mass spectrometer,supplemented by biochemical analysis and liver histopathological examination.Results Compared with the control group,the high-dose group,the medium dose group and the low-dose group of the ethanol extract of Inonotus Obliquus showed different degrees of inflammatory cell infiltration in the tissues.In the high-dose group,the liver of animals showed histopathological changes such as central venous congestion,bile duct hyperplasia and hepatocyte swelling.The content of serum albumin(ALB)could be decreased in the high-dose group and the medium dose group,the content of serum alanine aminotransferase(ALT)could be increased in the medium dose group,and the levels of serum bilirubin(TBIL),ALT and aspartate aminotransferase(AST)could be increased in the high-dose group.Through metabolomics research,30 differential biomarkers were identified in liver samples which were involved in the regulation of 7 metabolic pathways,among which steroid hormone biosynthesis and primary bile acid biosynthesis were the main metabolic pathways.Conclusion Ethanol extract of Inonotus Obliquus may promote the production of inflammatory factors through changes of steroid hormone biosynthesis and primary bile acid biosynthesis,leading to certain degree of liver injury in SD rats.This provides the reference for further research on the mechanism of liver injury caused by ethanol extract of Inonotus Obliquus.
ethanol extract of Inonotus Obliquusliver metabolomicsliver injurybiomarkerprimary bile acid
万方数据
维普
