In red clover (Trifolium pratense) there exists a soluble polyphenol oxidase (PPO) , which oxidizes native phenols to quinines, could bond to proteins, and inhibits proteolysis both in the silo and in the rumen. An observation of the red clover polyphenol oxidase indicated that substrate of PPO was o-diphenol, but not hydroquinone, m-diphenol, or mono-phenol. There were obvious differences between several substrates, and caffeine acid was the highest one for PPO activity. Maximum PPO activities of red clover showed pH 6. 5-7. 0, and the optimum temperature for the maximum PPO activity was 25-30℃, and heat stability was performed at 50 ℃.
Red clover (Trifolium pratense)Polyphenol oxidaseActivity
NETL
NSTL
维普
万方数据
