Physiological Response of Hard Seeds of Alfalfa on Accelerated Aging Duration
Studies were conducted with five alfalfa (Medicago sativa) seed with diverse hardness levels after accelerated aging at 24～96 h. This study compares seed germination; physiological and biochemical characteristics, and determines internal physiological factors inducing seed hardness affects to seed quality.Results show that both seed germination rate and electronical conductivity increase after aging treatment.Cell membrane permeability is increased with accelerated aging, while the hardness of seed is broken. For MS-1 sample (highest hardness), germination rate, superoxide dismutase (SOD), catalase (CAT) and Malonaldehyde (MDA) decrease with aging, whereas electronical conductivity increases. After aging 72h,germination rate, electronical conductivity, CAT and SOD contents decrease, whereas MDA contents increase. For MS-4 sample (less hardness), both electronical conductivity and MDA contents increase,whereas CAT and SOD levels decrease after 48h of accelerated aging. Harder seeds have stronger resistances, but higher seed germination rate does not mean higher seed vigor.
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