Isozyme Analysis of Different Chinese Iris Populations
Both peroxidase (POD) and esterase (EST) isozyme patterns of 23 Iris lactea var.chinensis (Fisch.) Koidz.accessions were studied using the vertical polyacrylamide gel electrophoresis in order to demonstrate their blood relationship and genetic diversity.Results indicated that eight general enzyme bands and fifteen specific enzyme bands at forty isozyme loci (Rf from 0.041 to 0.875 loci) were obtained.All tested Chinese Iris had homology, but genetic variation was occurred in some accessions.The lowest genetic similarity (0.3726) was observed between No.8 (BJCY-ML013) and No.19 (BJCY-ML028) showing their farther genetic relationship.The highest genetic similarity (0.9839) was observed among No.14 (BJCY-ML022), No.17 (BJCY-ML026), No.13 (BJCY-ML021) and No.18 (BJCY-ML027) indicating their closer genetic relationships.The genetic similarities of other accessions were from 0.4049 to 0.9678 indicating that all tested Chinese Iris had close genetic relationships.Cluster analysis showed that the 23 wild Chinese Iris accessions were classified into 5 groups as the similarity coefficient was 0.79.The germ-plasm resources from similar geographical habitats were clustered into one group indicating the correlation between all tested 23 Chinese Iris accessions and their geographical habitats.
Iris lactea var. Chinensis (Fisch.) Koidz.PODESTGenetic similarity
NETL
NSTL
万方数据
维普
