Cloning and Expression Analysis of Partial Fragments from Lignin Biosynthesis F5H Genes in Switchgrass
Switchgrass (Panicum virgatum L. ) is an important lignocellulosic crop. High lignin content seriously limits its utilization as an energy plant. The partial fragments of F5H genes involved in G/S lig-nin monomer conversion were amplified from switchgrass bark mRNA using RT-PCR and designated as PvFoHl and PvF5H2 respectively (GenBank accession number: JQ430747 and JQ430748). DNAMAN sequence analysis of both genes indicated that the similarity of nucleic acid sequence reached 93% and the similarity of protein sequence reached 90%. NCBI Blast online was performed to analyze the biological in-formation of both PvF5Hl and PvF5H2. Sequence analyzed through biological methods showed that en-coded polypeptides were highly homologous to F5H proteins of Zea mays. Phylogenetic tree analysis also demonstrated that both genes were related to the F5H gene from Zea mays. Real-time quantitative PCR (qPCR) was performed to investigate the expression profiles of both PvF5Hl and PυFSHZ under wound stress. The results of qPCR analysis revealed that both PυF5H1and PυF5H2 showed fast-response and up-regulated expression under wound stress. PυF5H1 and PυF5H2 had different expression level with similar changing tendency.
NETL
NSTL
万方数据
维普
