Effects of Different Mutagenic Treatments on the Polyploid Induction of Miscanthus sinensis
Miscanthus Anderss is a new kind of lignocellulosic biomass for the production of biofuels.In order to improve the biomass production and resistance,the polyploid induction of Miscanthus have been widely researching all over the world.The purpose of this study is to investigate the effects of different treatments on the polyploid intrduction of Miscanthus Anderss.The DNA content of plant leaves is measured by flow cytometry instrument,the chromosome of roots is identified using chromosome technique,and the stomatal guard cells are compared between tetraploid and diploid.The results show that soaking callus in 10 μmol · L 1oryzalin solution with 1.02 mmol · L-1 Ca2+ for 15 minute at 5℃ (35.19％) is the best method for polyploidy induction.The colchicine has higher toxicity than the others,and there is no significant difference between oryzalin and nocodazole.
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