Effects of Magnetized Water on the Growth and Nitrogen Fixation of Rhizobial Strain on Alfalfa
The effects of magnetized water treatment on the growth and nitrogen fixation of rhizobial strain on alfalfa under the constant magnetic field of 20000 Gs were investigated.The results showed that magnetization significantly affected the growth of XGL026 and reduced the OD value.The counts of viable bacteria in magnetized water culture media were the 86.96% ~65.72% of ordinary culture media,and the counts of viable bacteria decreased with the times of magnetization increasing.The water magnetized more than three times significantly reduced the counts of viable bacteria (P <0.01).Pot alfalfa was inoculated by the marked bacteria strain XGL026-GFP.The irrigation of magnetized water had inhibitory effect on the colonization and survival of the rhizobial in alfalfa rhizosphere and reduced 46.4% nodule number compared to ordinary water (P<0.01).Both above-and below-ground of alfalfa were influenced by magnetized water treatment,the rates of N fixation decreased 21.06% and 20.48%,the amount of N fixation decreased by 26.64% and 37.5%,the dry biomasses were reduced by 27.62% and 13.46%,respectively.Thus the magnetized water treatment had effects on the growth,colonization and nitrogen fixation of rhizobial strain in alfalfa rhizosphere.
RhizobiaMagnetized waterBiological effectThe proportion of N fixed