Study on Differential Proteomics of Leymus chinensis Under Saline-alkaline Stress
To reveal the molecular mechanism of saline-alkaline tolerance from Leymus chinensis,the protein expression profile was constructed by 2D-DIGE and MALDI TOF/TOF.There were 74 protein spots presenting to express differentially after the total proteins were separated by 2D-DIGE.The 44 proteins of all presenting spots were up-regulated,and 30 proteins were down-regulated.Among the 74 protein spots,the annotations of the 33 differential expressed proteins were known.The expressed proteins were analyzed and compared by mass spectrum.Identified differential proteins took part in the energy exchange,metabolic processes of basal metabolism,photosynthesis,plant defense,and DNA synthesis,respectively.These works will be fundamental data to illuminate the molecular mechanism of plant defense to saline-alkaline stress.