Effect of Storage Temperature on Vigor of Sweet Clover Seed under Different Moisture Content Conditions
The experiments were conducted to study the influence of storage temperature on vigor index in sweet clover seeds.The results showed that:storage temperature and moisture content significantly effect on sweet clover seed germination rate,conductivity,soluble sugar and MDA content (P<0.01),the interaction of temperature and moisture content also had a very significant effect on sweet clover seed germination rate,conductivity,soluble sugar content (P<0.01).With the increase of sweet clover seed moisture content and storage temperature,the germination rate first increased and then decreased.When moisture content was 10% and storage temperature was 4℃,sweet clover seed germination rate was highest.In contrast,when moisture content was 12% and storage temperature was 20℃,sweet clover seed germination rate was dropped to 0;with the increase of sweet clover seed moisture content and storage temperature,the electrical conductivity and soluble sugar content first increased and then decreased.When moisture content was 12% and storage temperature was 20℃,they both reached the highest;with the increase of sweet clover seed moisture content,the MDA content increased.But,with the increase in storage temperature,the MDA content decreased.The correlation analysis demonstrated that sweet clover seed conductivity and soluble sugar content showed a significant negative linear relationship with germination rate (P<0.01).Integrated the vitality index,the optimum storage temperature of sweet clover seed was 4℃,the optimum moisture content was 10 %.
Sweet clover seedStorage temperatureSeed moisture contentSeed vigor index