Research Progress in Protoplast Culture and Fusion Technologies of Gramineous Forage and Turfgrass Plants
Gramineous forage and turf grass plants play important roles in supporting the sustainable agriculture developments,afforestation of city and environmental protection.Somatic hybridization,which has great potential in transferring the target characters controlled by multigenes or uncloned genes,is an important supplement to the sexual hybridization.In this paper,the technologies of protoplast culture and fusion of gramineous forage and turf grass were reviewed.It included selecting the materials for protoplast isolation,culture media,culture density and methods,fusion methods.Moreover,the prospects of the protoplast culture and fusion technologies of gramineous forage and turf grass plants were made.