首页|PEG引发对老化燕麦种子抗氧化性能的影响

PEG引发对老化燕麦种子抗氧化性能的影响

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试验以45℃老化48 d的超干(含水量4%)燕麦(Avena sativa)种子为材料,通过不同浓度PEG-6000(0,-0.3,-0.6,-0.9和-1.2 Mpa)引发0(CK),3和6h后,分析其超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性及丙二醛(MDA)含量的变化,以探讨PEG引发对老化的超干燕麦种子抗氧化性能的影响.结果表明:浓度为0和-0.3 Mpa的PEG引发会降低SOD,CAT和POD的活性,导致MDA含量增加,从而降低老化燕麦种子发芽率;而高浓度(-0.9和-1.2 Mpa)的PEG引发则相反.说明适当浓度的PEG引发主要通过提高超干燕麦种子的抗氧化能力来修复其老化损伤,并避免其吸胀损伤.
Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds
This study was designed to analyze the activities of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD),and malondialdehyde (MDA) content in ultra-dried (4% moisture content) oat (Avena sativa) seeds.The seeds were deteriorated at 45C for 48 d,and then primed with different concentrations of PEG-6000 (0,-0.3,-0.6,-0.9 and-1.2 Mpa) for 0 (CK),3 and 6 h.This study was to explore the effect of PEG priming on physiological responses of aged ultra-dry oat seeds.The results showed that the activities of SOD,CAT and POD decreased in aged oat seeds primed with PEG at the concentration of 0 and-0.3 Mpa,leading to an increase in MDA content and decrease in germination percentage.However,the opposite trend was observed after priming with high concentration of PEG (-0.9 and-1.2 Mpa).This indicated that PEG priming repaired the damage of ageing and prevented the imbibition damage in ultra-dried oat seeds by improving antioxidant capacity.

OatPrimingPolyethylene glycolSeed ageingUltra-dry

夏方山、毛培胜、王明亚、闫慧芳、侯龙鱼、陈玲玲、朱艳乔、汪辉

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山西农业大学动物科技学院,山西太谷030801

中国农业大学动物科技学院草业科学北京市重点实验室,北京100193

燕麦 引发 聚乙二醇 种子老化 超干

博士点基金国家“十二五”科技支撑课题

201100081100032011BAD17B01-02

2016

草地学报
中国草学会

草地学报

CSTPCDCSCD北大核心
影响因子:1.156
ISSN:1007-0435
年,卷(期):2016.24(5)
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