Effects of Transcriptional Regulation of MsACS2 by MsWRKY33 on Salt Tolerance of Alfalfa
Ethylene,as one of the important plant hormones,plays an important role in plant stress re-sponse.The ACS gene is a key rate limiting enzyme(ACC synthase)gene in the ethylene synthesis process.In this study,we laid a theoretical foundation for understanding the specific regulatory mechanism of MsWRKY33 participating in salt-tolerant response of alfalfa by exploring the regulatory relationship of MsWRKY33 transcription factor on ACS gene.We used homologous cloning,vector construction,Y1H,Y2H,qRT-PCR and other techniques to verify the transcriptional regulation of MsWRKY33 on AtACS2 and MsACS2,meanwhile,analyzed the expression pattern of MsACS2 in transgenic lines under salt stress.The results showed that the MsWRKY33 transcription factor could specifically bind to the W-box element and had transcriptional regulatory effects on AtACS2 and MsACS2;The expression level of MsWRKY33 gene in the transgenic lines was significantly higher than that in the control.The expression of MsACS2 in transgenic lines showed an increasing trend after the large amount of MsWRKY33 gene expression,further indicating that the expression of MsACS2 gene in alfalfa might be positively regulated by the MsWRKY33 transcription factor.The results indicated that the expression of MsWRKY33 could be induced when alfal-fa was subjected to salt stress.The MsWRKY33 transcription factor might affect ethylene synthesis and salt stress response of alfalfa by positively regulating the expression of MsACS2.
万方数据
维普
