Characterization and Analysis of Gibberellin Insensitive Dwarf1 Gene Family and Response to Abiotic Stresses in Tall Fescue
Gibberellin receptor(Gibberellin insensitive dwarf1)can sense the signal of gibberellin and par-ticipate in the regulation of plant growth and development,as well as in plant abiotic stress resistance.Tall fescue(Festuca arundinacea)is a low-maintenance cool-season turfgrass and is exposed to various stres-ses,but the role of its gibberellin receptor family in stress and development has not been reported yet.In this study,12 gibberellin receptor gene sequences were identified in the transcriptome of tall fescue,which contained highly conserved domains and could be divided into two clades,GID1L2(gibberellin receptor 1 like 2)for class I and GID1 for class Ⅱ.The expression patterns of these genes in crown and leaf of tall fescue under low temperature stress(0℃),high temperature stress(38℃),salt stress(120 mmol·L-1 NaCl)and short-term drought(15％PEG 6000)were determined by quantitative real-time polymerase chain reaction.The results showed that these genes were responsive to various abiotic stresses.The ex-pression patterns of FaGID1L2 genes were different in crown and leaf,but FaGID1s'were similar.Except the expression pattern of FaGID1d in leaf under cold stress was unique,the expression pattern of the rest FaGID1s under cold stresses was consistent.These results provided a theoretical basis for further func-tional analysis of gibberellin receptor genes involved in stresses.
万方数据
维普
