To explore the molecular mechanisms and metabolic pathways of genes related to the symbiotic relationship between'Mengnong Clover No.1'(T.ambiguum Bieb.'Mengnong No.1')and rhizobia,this study selected'Mengnong Clover No.1'inoculated with rhizobia and an uninoculated control group as research subjects.Through transcriptomic sequencing,we compared the transcriptome differences between symbiotic and non-symbiotic states.The results showed that,compared with the uninoculated group,a to-tal of 1105 differentially expressed genes(DEGs)were identified with significant changes of expression levels in all symbiotic samples.Among them,677 genes were upregulated and 428 genes were downregulat-ed.The reliability of the transcriptomic sequencing was confirmed by real-time quantitative PCR(qRT-PCR)for some of these genes.Further analysis revealed that these DEGs were involved in multiple meta-bolic pathways and biological processes,including biosynthesis of phenylpropanoids,metabolism of amino sugars and nucleotide sugars,as well as cysteine and methionine metabolism.This study reveals the tran-scriptomic characteristics of'Mengnong Clover No.1'under symbiotic conditions with rhizobia,and pro-vides important references for a deeper understanding of the symbiotic mechanisms between plants and rhi-zobia.
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