Screening of Alkaloid-producing Endophytic Fungi from Sophora alopecuroides and Optimization of their Fermentation Conditions
The aim of this study was to screen the endophytic fungal strains of Sophora alopecuroides that can produce Quinolizidine alkaloid,to explore the fermentation conditions and improve alkaloid production.Fifty endophytic fungal strains isolated from healthy seeds of S.alopecuroides in the previous period were used as materials.The alkaloid-producing strains were screened by Thin-Layer Chromatography,High-Performance Liquid Chromatography and Acid-dye colorimetry.The alkaloid-producing strains obtained were analyzed by morphology and multi-gene sequence comparison to determine the taxonomic status.Subsequently,the effects of the addition of precursors such as L-pipericolic acid,L-lysine and α-ketoglutaric acid on the alkaloid yields of alkaloid producing strains were explored by one-way and response surface methodology.The results showed that a total of three highly active Sophoridine-producing strains,DSD103,DSD201 and DSD308,all belong-ing to Alternaria alternata,were screened,and the alkaloid yields of Sophoridine in their mycelia were 0.212,0.544 and 0.276 mg·g-1.The highest alkaloid yielding strain,DSD201,was obtained with the addition of the pre-cursors L-pipericolic acid at a concentration of 1.079 μmol·L-1,L-lysine at a concentration of 6.251 μmol·L-1,and α-ketoglutaric acid at a concentration of 0.153 μmol·L-1.Under this conditions,the alkaloid yield in myce-lium reached the maximum of 0.949 mg·g-1,which was 74.12% higher than that under the control conditions.The screened alkaloid-producing strains provide a new method for the production of Quinolizidine alkaloid by microbial fermentation.
Endophytic fungi of Sophora alopecuroidesAlkaloid-producing strainsScreeningOptimization of fermentation conditionsQuinolizidine alkaloid
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维普
