首页|绿原酸对柱花草青贮品质、蛋白降解和细菌群落及相互关系的影响

绿原酸对柱花草青贮品质、蛋白降解和细菌群落及相互关系的影响

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为改善柱花草(Stylosanthes)的青贮发酵品质,将0。2%和0。4%的绿原酸添加到柱花草中,以无添加作为对照(CK)。青贮80 d后,分析柱花草的发酵特性、蛋白组成以及细菌群落变化,结果表明:添加绿原酸不仅显著降低了柱花草的pH值、非蛋白氮、氨态氮含量,对干物质、乳酸和乙酸含量的变化均有积极的影响。细菌群落分析表明添加绿原酸对芽孢杆菌、魏斯氏菌,对肠杆菌、泛菌、丁酸梭菌的含量有较好的抑制作用。此外,0。2%绿原酸处理还显著降低了丙酸含量以及酸性蛋白酶和氨基肽酶的活性。综上所述,添加绿原酸对柱花草的发酵品质、蛋白组分均有积极的作用。
The Effects of Chlorogenic Acid on the Quality,Protein Degradation,Bacterial Community,and Their Interrelationships of Stylosanthes Silage
To improve the fermentation quality of silage of Stylosanthes,0.2% and 0.4% chlorogenic acid were added to Stylosanthes,with no addition as the control(CK).After 80 days of ensiling,the fermentation charac-teristics,protein composition,and bacterial community changes of Stylosanthes were analyzed.The results showed that the addition of chlorogenic acid not only significantly reduced the pH value,non protein nitrogen,and ammonia nitrogen content of Stylosanthes,but also had a positive impact on the changes of lactic acid,dry matter and acetic acid content.Bacterial community analysis showed that the addition of chlorogenic acid had a good inhibitory effect on the content of Bacillus and Weissella,as well as Enterobacterium,Pantobacterium,and Clostridium butyricum.In addition,0.2% chlorogenic acid treatment significantly reduced the content of propi-onic acid and the activity of acid protease and aminopeptidase.In summary,adding chlorogenic acid had a posi-tive effect on the fermentation quality and protein components of Stylosanthes.

StylosanthesChlorogenic acidProteaseSilage qualityBacterial community

王垚、郭田心、陈丹丹、梁秋雨、张庆

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华南农业大学林学与风景园林学院/广东木本饲料工程技术研究中心/广东省森林植物种质创新与利用重点实验室,广东 广州 510642

柱花草 绿原酸 蛋白酶 青贮品质 细菌群落

2025

草地学报
中国草学会

草地学报

北大核心
影响因子:1.156
ISSN:1007-0435
年,卷(期):2025.33(1)