Effects of Various Alfalfa-Crop(Forage)Rotation Patterns on Bacterial Community Composition and Ecological Function Prediction
In this study,seven grass-grain(grass)crop rotation patterns were selected,and the effects of differ-ent rotation patterns on soil nutrient content and microbial diversity were investigated with the help of high-throughput sequencing technology.The results showed that compared with alfalfa continuous cropping mode,alfalfa-oats-alfalfa,alfalfa-corn-flax and alfalfa-sorghum-millet models effectively increased the contents of total phosphorus,available phosphorus,total potassium and available potassium in soil,and the bacterial com-munity diversity index was the highest in alfalfa-sorghum-millet model.Proteobacteria,Acidobacteriota and Actinobacteriota were the dominant bacterial groups in soil bacterial communities under different cropping pat-terns.Further redundancy analysis revealed that organic carbon,total nitrogen and available nitrogen had more significant effects on soil bacterial community composition.The results of ecological function prediction showed that Metabolism was the most important function of soil bacteria metabolism.In conclusion,the bacterial com-munity structure and ecological function of soil were changed after perennial alfalfa was turned into annual crop(forage).Among them,alfalfa-sorghum-millet model had the best effect,and could be given priority in the improved rotation of saline-alkali land.
AlfalfaCrop rotationSoil bacterial communityEcological function prediction