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雪胆的鉴别及含量测定

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目的:建立雪胆的鉴别及含量测定方法,为雪胆药材质量标准的制定提供科学依据。方法:采用显微鉴别法、薄层色谱法对雪胆进行定性鉴别,采用高效液相色谱法对雪胆甲素进行定量分析。结果:显微鉴别和薄层色谱结果表明雪胆有较显著的鉴别特征。HPLC法表明雪胆甲素峰面积与进样量在0. 1255 mg/mL~2. 51 mg/mL呈良好线性关系,可为评价雪胆品质提供依据。结论:所建立的方法简便、准确、可靠,重现性好,可为提供雪胆的鉴别及含量测定参考。
Study on Quality Standard of Hemsleya Amabilis
Objective: To provide scientific basis for the utilization and development of Hemsleya Amabilis by establishing the I-dentification and determination method. Method: The qualitation identification was carried out by microscopic identification and TLC. Arid quantitation determination was carried out by HPLC. Result: The results of microscopic identification and TLC showed that significant characteristics were found for Hemsleya Amabilis. The results of HPLC quantitative analysis showed that the linear range of rubi-maillin was 0.1255 mg ? mL1 'l] - 2. 51mg ? mL[ ~1]. Conclusion: These methods are convenient, accurate, reliable with good re producibility and can be used to enhance the quality standard for the medicinal material.

Hemsleya Amabilismicroscopic identificationTLCHPLC

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四川省绵阳市食品药品检验所,四川绵阳621000

雪胆 显微鉴别 薄层色谱 高效液相色谱

四川省中药材标准修订起草项目

川食药监注[2008]21号

2011

成都中医药大学学报
成都中医药大学

成都中医药大学学报

CSTPCD
影响因子:0.572
ISSN:1004-0668
年,卷(期):2011.(3)
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