Gallic Acid in Improving Hypoxia-induced BV2 Microglia Injury via Inhibiting NF-κB Pathway
Objective:To establish hypoxia injury model of BV2 microglia,investigating the gallic acid in improving hypoxia-induced BV2 microglia injury.Methods:BV2 microglia were divided into blank group,model group,low-dose,medium-dose and high-dose groups.Hypoxia injury model of BV2 microglia was established in a three-gas incubator.Low-dose,medium-dose and high-dose groups were treated with gallic acid of 1.7 g/mL、3.4 g/mL、8.5 g/mL.CCK-8 method was use to detected the protective effect of gallic acid on hypoxia-induced BV2 microglia injury.LDH viability in supernatant,SOD activity,MDA and ROS content in cells were detected using the kit.Inflammatory factors(TNF-α、IL-1β、IL-6)in supernatant were detected by ELISA kits.The expression level of p-IKK/IKK,p-IκB/IκB and p-NF-κB p65/NF-κB p65 and nuclear displacement of NF-κB p65 in BV2 microglia were detected by Western blot.Results:Compared with control group,hypoxia can increase LDH activity and inflammatory factors(TNF-αIL-1βIL-6)in superna-tant,decrease SOD activity and increased MDA and ROS content in in cells,and NF-κB pathway(P-IKK/IKK P-I κB/IκB and P-NF-κB P65/NF-κB p65 protein expression level)and nuclear displacement of NF-κB p65.Gallic acid can improve hypoxia-induced BV2 microglia injury via inhibiting oxidative stress(Increase SOD activity,decrease MDA and ROS content)and the release of inflammatory factors,inhibiting the activation of NF-κB pathway and nuclear displacement of NF-κB p65.Conclusion:Gallic acid has a protective effect on hypoxia-induced BV2 microglia injury.
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