Objective:To explore the effect of Danba soaking process on aconite alkaloids in the processing of Fuzi,and determine the best process parameters.Methods:A single factor investigation was carried out from the three aspects of the concentration of the Dan-ba aqueous solution,the soaking time of the Fuzi in the Danba aqueous solution,and the solid-liquid ratio of the aconite and the Danba aqueous solution.The HPLC method was used to determine the content of the Fuzi according to the ＂Chinese Pharmacopoeia＂2020 e-dition.The alkaloid content in the aconite was determined,and the change of the alkaloid content in the aconite was analyzed.Re-sults:The content of diester alkaloids in Fuzi showed a general downward trend with the prolongation of bile infusion time,the decrease of the solid-liquid ratio of bile solution and the increase of the concentration of bile solution;the content of monoester alkaloids had no obvious change;The total alkaloid content showed a downward trend.Based on the morphology and alkaloid content of Fuzi,the best process for soaking Danba was determined to be 35%-40%Danba aqueous solution concentration,and the aconite soaked at a solid-liquid ratio of 1∶2 for no less than 20 days.Conclusion:Danba soaking can effectively preserve Fuzi and reduce the content of its toxic components,diester-type alkaloids.The optimal soaking process can provide a reference for the processing of Fuzi in Danba.
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