Study on Geoherbalism of Atractylodis Rhizome Based on Gut Microbiota
Objective:The anti-diarrhea effect of Atractylodis Rhizome from different areas was studied based on the gut microbiota,which provides the basis for the geoherbalism research of Atractylodis Rhizome.Methods:The diarrhea model was induced by rhubarb aqueous extract(10 g/kg).Then mice were treated with Atractylodis Rhizome aqueous extract(1.85 g/kg)from different areas for 7 days.The status of the mice was observed,and fecal score,fecal water content,small intestinal propulsion rate,and pathological changes and the expression of AQP3 and AQP4 in the colon.The changes of gut microbiota in mice were observed by 16S rRNA se-quencing.Results:Compared with the model group,both Atractylodis Rhizome from genuine producing areas and non-genuine produ-cing areas could significantly improve the phenotypic indicators of diarrhea mice.Furthermore,Atractylodis Rhizome from genuine pro-ducing areas(Jiangsu)has a better inhibitory rate on small intestinal propulsion in mice with diarrhea than Atractylodis Rhizome from non-genuine producing areas(Hubei),and its ability to increase AQP3 and AQP4 is better than Atractylodis Rhizome from non-genu-ine producing areas(Hebei and Hubei).In addition,16S rRNA sequencing analysis showed Atractylodis Rhizome from genuine pro-ducing areas and non-genuine producing areas could improve beta diversity of gut microbiota.Species composition of gut microbiota showed that Atractylodis Rhizome from genuine producing areas(Jiangsu)and non-genuine producing areas(Hebei and Hubei)re-duced the abundance of Firmicutes while increased the abundance of Bacteroidota.The distinction is that Atractylodis Rhizome from genuine producing areas(Jiangsu)can diminish Actinobacteriota abundance while increasing Verrucomicrobia;Atractylodis Rhizome from non-genuine producing areas(Hubei)increased Verrucomicrobia.At the genus level,Atractylodis Rhizome from genuine produ-cing areas(Jiangsu)and non-genuine producing areas(Hebei and Hubei)regulated distinct species,with genuine producing areas(Jiangsu)having the most regulated species.Conclusion:These findings demonstrated that the anti-diarrheal effect of Atractylodis Rhizome from genuine producing areas(Jiangsu)is better than that of Atractylodis Rhizome from non-genuine producing areas(Hebei and Hubei)had anti-diarrheal effects,which may be due to the stronger ability of Atractylodis Rhizome from genuine producing areas to regulate gut microbiota.
万方数据
维普
